![]() ![]() Many tools facilitate the comparison of traces with a short reference subsequence, but they lack support to align traces across entire genomes, to deconvolute mutations or to patch a reference sequence based on trace information. Sanger sequencing has a long history in molecular biology and it remains indispensable for many routine tasks like the sequencing of single genes, cloned plasmids, expression constructs or PCR products. Molecular biologists benefit from the companion web applications that enable installation-free Sanger chromatogram analyses using intuitive, graphical user interfaces. Tracy can be routinely applied in large-scale validation efforts conducted in clinical genomics studies as well as for high-throughput genome editing techniques that require a fast and rapid method to confirm discovered variants or engineered mutations. The software is open-source and freely available at, the companion web applications are hosted at. Tracy can be easily integrated in large-scale pipelines and high-throughput settings, and it uses state-of-the-art file formats such as JSON and BCF for reporting chromatogram sequencing results and variant calls. Its companion web applications make all functionality of Tracy easily accessible using standard web browser technologies and interactive graphical user interfaces. We present Tracy, an efficient and versatile command-line application that enables basecalling, alignment, assembly and deconvolution of sequencing chromatogram files. ![]() Despite its long history, there is a lack of user-friendly Sanger sequencing data analysis tools that can be run interactively as a web application or at large-scale in batch from the command-line. DNA sequencing is at the core of many molecular biology laboratories. ![]()
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